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qpcr greenmaster mix  (Jena Bioscience)


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    Structured Review

    Jena Bioscience qpcr greenmaster mix
    Qpcr Greenmaster Mix, supplied by Jena Bioscience, used in various techniques. Bioz Stars score: 95/100, based on 142 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/qpcr+greenmaster+mix/qPCR+GreenMaster/pmc13106029-156-5-8
    Average 95 stars, based on 142 article reviews
    qpcr greenmaster mix - by Bioz Stars, 2026-09
    95/100 stars

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    Related Articles

    Quantitative RT-PCR:

    Article Title: Cross-kingdom RNA interference promotes arbuscular mycorrhiza development.
    Article Snippet: The RNA was treated with Invitrogen DNAse I amp. grade (www.invitrogen.com) and tested for purity via PCR. cDNA synthesis was performed with 1 μg RNA using SuperScript IV Reverse Transcriptase (Invitrogen) and the 3′ Oligo-dT primer. .. Real-time RT-qPCR was performed with qPCR GreenMaster Mix ( Jena Bioscience) and with the primers shown in Supplementary Table 1. .. The qPCR reaction was run on a LightCycler 480 II (Roche).

    Article Title: Cross-kingdom RNA interference promotes arbuscular mycorrhiza development
    Article Snippet: The RNA was treated with Invitrogen DNAse I amp. grade ( www.invitrogen.com ) and tested for purity via PCR. cDNA synthesis was performed with 1 μg RNA using SuperScript IV Reverse Transcriptase (Invitrogen) and the 3′ Oligo-dT primer. .. Real-time RT-qPCR was performed with qPCR GreenMaster Mix (Jena Bioscience) and with the primers shown in Supplementary Table . .. The qPCR reaction was run on a LightCycler 480 II (Roche).

    Real-time Polymerase Chain Reaction:

    Article Title: Cross-kingdom RNA interference promotes arbuscular mycorrhiza development.
    Article Snippet: The RNA was treated with Invitrogen DNAse I amp. grade (www.invitrogen.com) and tested for purity via PCR. cDNA synthesis was performed with 1 μg RNA using SuperScript IV Reverse Transcriptase (Invitrogen) and the 3′ Oligo-dT primer. .. Real-time RT-qPCR was performed with qPCR GreenMaster Mix ( Jena Bioscience) and with the primers shown in Supplementary Table 1. .. The qPCR reaction was run on a LightCycler 480 II (Roche).

    Article Title: Cross-kingdom RNA interference promotes arbuscular mycorrhiza development
    Article Snippet: The RNA was treated with Invitrogen DNAse I amp. grade ( www.invitrogen.com ) and tested for purity via PCR. cDNA synthesis was performed with 1 μg RNA using SuperScript IV Reverse Transcriptase (Invitrogen) and the 3′ Oligo-dT primer. .. Real-time RT-qPCR was performed with qPCR GreenMaster Mix (Jena Bioscience) and with the primers shown in Supplementary Table . .. The qPCR reaction was run on a LightCycler 480 II (Roche).

    Article Title: Circulating ESR1, long non-coding RNA HOTAIR and microRNA-130a gene expression as biomarkers for breast cancer stage and metastasis
    Article Snippet: Real-time PCR: TaqMan ® Gene Expression Master Mix (Applied Biosystems, Cat. No. 4369016) and TaqMan ® assays (for hsa-miR-130a 20× and TBP 20×) were used for real-time PCR. .. For ESR1, HOTAIR, and GAPDH, qPCR GreenMaster Mix (Jena Bioscience, Cat. No. PCR-336S) and specific primers (Sangon Biotech Co., Ltd., Shanghai, China) were employed. ..

    Article Title: Circulating ESR1, long non-coding RNA HOTAIR and microRNA-130a gene expression as biomarkers for breast cancer stage and metastasis.
    Article Snippet: .. Real-time PCR: (a) TaqMan® Gene Expression Master Mix (Applied Biosystems, Cat. No. 4369016) and TaqMan® assays (for hsa-miR-130a 20× and TBP 20×) were used for real-time PCR. (b) For ESR1, HOTAIR, and GAPDH, qPCR GreenMaster Mix (Jena Bioscience, Cat. No. PCR-336S) and specific primers (Sangon Biotech Co., Ltd., Shanghai, China) were employed. ..

    Article Title: Bite through the tent.
    Article Snippet: The authors report on a young boy who was bitten into his face by an unknown animal while being asleep in a tent.. Given the bite marks and the location of the scene, members of the mustelidae and canidae families were the first “suspects.” Deoxyribunucleic acid (DNA) recovered from the tent’s wall was analyzed with regard to parts of the mitochondrial 12S ribosomal ribunucleic acid (12S rRNA) and cytochrome b (cytb) genes as well as nuclear short tandem repeats (STRs).. Since Sanger sequencing revealed a mixed sequence with a strong human component overlying the nonhuman contributor, an animal screening using a duplex real-time polymerase chain reaction (PCR) with an intercalating dye and melt curve analysis was employed.

    Polymerase Chain Reaction:

    Article Title: Circulating ESR1, long non-coding RNA HOTAIR and microRNA-130a gene expression as biomarkers for breast cancer stage and metastasis
    Article Snippet: Real-time PCR: TaqMan ® Gene Expression Master Mix (Applied Biosystems, Cat. No. 4369016) and TaqMan ® assays (for hsa-miR-130a 20× and TBP 20×) were used for real-time PCR. .. For ESR1, HOTAIR, and GAPDH, qPCR GreenMaster Mix (Jena Bioscience, Cat. No. PCR-336S) and specific primers (Sangon Biotech Co., Ltd., Shanghai, China) were employed. ..

    Article Title: Circulating ESR1, long non-coding RNA HOTAIR and microRNA-130a gene expression as biomarkers for breast cancer stage and metastasis.
    Article Snippet: .. Real-time PCR: (a) TaqMan® Gene Expression Master Mix (Applied Biosystems, Cat. No. 4369016) and TaqMan® assays (for hsa-miR-130a 20× and TBP 20×) were used for real-time PCR. (b) For ESR1, HOTAIR, and GAPDH, qPCR GreenMaster Mix (Jena Bioscience, Cat. No. PCR-336S) and specific primers (Sangon Biotech Co., Ltd., Shanghai, China) were employed. ..

    Gene Expression:

    Article Title: Circulating ESR1, long non-coding RNA HOTAIR and microRNA-130a gene expression as biomarkers for breast cancer stage and metastasis.
    Article Snippet: .. Real-time PCR: (a) TaqMan® Gene Expression Master Mix (Applied Biosystems, Cat. No. 4369016) and TaqMan® assays (for hsa-miR-130a 20× and TBP 20×) were used for real-time PCR. (b) For ESR1, HOTAIR, and GAPDH, qPCR GreenMaster Mix (Jena Bioscience, Cat. No. PCR-336S) and specific primers (Sangon Biotech Co., Ltd., Shanghai, China) were employed. ..

    Screening Assay:

    Article Title: Bite through the tent.
    Article Snippet: The authors report on a young boy who was bitten into his face by an unknown animal while being asleep in a tent.. Given the bite marks and the location of the scene, members of the mustelidae and canidae families were the first “suspects.” Deoxyribunucleic acid (DNA) recovered from the tent’s wall was analyzed with regard to parts of the mitochondrial 12S ribosomal ribunucleic acid (12S rRNA) and cytochrome b (cytb) genes as well as nuclear short tandem repeats (STRs).. Since Sanger sequencing revealed a mixed sequence with a strong human component overlying the nonhuman contributor, an animal screening using a duplex real-time polymerase chain reaction (PCR) with an intercalating dye and melt curve analysis was employed.



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    Positive correlation between m6A modification levels and replication capacity of NiV strains. ( A ) NiV RNA copy numbers in supernatants collected at various time points post-infection were quantified by absolute <t>qPCR.</t> Data are means G SEMs ( n = 3). ** p < 0.01, two-way ANOVA with Holm–Sidak’s multiple comparisons test. ( B ) Analysis of published data comparing the impact of NiV-M and NiV-B strains on hamster survival under identical treatment conditions. ( C ) UHPLC-MS/MS analysis of NiV RNA. Viral RNA from supernatants of NiV-infected Vero cells was purified using Dynabeads and analyzed by UHPLC-MS/MS. The y -axis shows m6A as a percentage of total adenosines. ( D ) MeRIP-qRT-PCR analysis. RNA from NiV-infected Vero cells was immunoprecipitated with anti-m6A antibodies, followed by strand-specific qRT-PCR to detect the NiV negative-strand genome. Data are presented as means ± SEMs ( n = 3). ** p < 0.01, unpaired Student’s t -tests. ( E ) MeRIP-Seq. Fragmented viral RNA from the supernatant of NiV-infected Vero cells was immunoprecipitated with an m6A-specific antibody and analyzed by next-generation sequencing. Methylation coverage of input and MeRIP-enriched NiV RNA is shown in blue and red, respectively. ( F ) Nanopore DRS analysis of NiV-M and NiV-B mRNAs from infected Vero cells. The y -axis shows m6A methylation probability at each A site; only sites with >95% probability are shown. Shared m6A sites between both strains are marked in red, while NiV-M- and NiV-B-specific sites are highlighted in green and yellow, respectively. ( G , H ) Bioinformatics analysis of m6A distribution and conserved motifs in NiV-M and NiV-B mRNAs. ( I ) MeRIP-qRT-PCR. RNA from NiV-infected Vero cells was immunoprecipitated with anti-m6A antibodies, followed by strand-specific qRT-PCR for NiV mRNA. Data are mean ± SEM ( n = 3); ** p < 0.01, unpaired Student’s t -test. ( J ) MeRIP-qRT-PCR analysis of m6A-modified viral RNA in lung tissues of NiV-infected hamsters. Total RNA was extracted from lung tissue, immunoprecipitated using anti-m6A antibodies, and subjected to qRT-PCR targeting NiV mRNA. Data are presented as mean ± SEM ( n = 4); * p < 0.05, unpaired Student’s t -test with Welch’s correction.
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    Positive correlation between m6A modification levels and replication capacity of NiV strains. ( A ) NiV RNA copy numbers in supernatants collected at various time points post-infection were quantified by absolute qPCR. Data are means G SEMs ( n = 3). ** p < 0.01, two-way ANOVA with Holm–Sidak’s multiple comparisons test. ( B ) Analysis of published data comparing the impact of NiV-M and NiV-B strains on hamster survival under identical treatment conditions. ( C ) UHPLC-MS/MS analysis of NiV RNA. Viral RNA from supernatants of NiV-infected Vero cells was purified using Dynabeads and analyzed by UHPLC-MS/MS. The y -axis shows m6A as a percentage of total adenosines. ( D ) MeRIP-qRT-PCR analysis. RNA from NiV-infected Vero cells was immunoprecipitated with anti-m6A antibodies, followed by strand-specific qRT-PCR to detect the NiV negative-strand genome. Data are presented as means ± SEMs ( n = 3). ** p < 0.01, unpaired Student’s t -tests. ( E ) MeRIP-Seq. Fragmented viral RNA from the supernatant of NiV-infected Vero cells was immunoprecipitated with an m6A-specific antibody and analyzed by next-generation sequencing. Methylation coverage of input and MeRIP-enriched NiV RNA is shown in blue and red, respectively. ( F ) Nanopore DRS analysis of NiV-M and NiV-B mRNAs from infected Vero cells. The y -axis shows m6A methylation probability at each A site; only sites with >95% probability are shown. Shared m6A sites between both strains are marked in red, while NiV-M- and NiV-B-specific sites are highlighted in green and yellow, respectively. ( G , H ) Bioinformatics analysis of m6A distribution and conserved motifs in NiV-M and NiV-B mRNAs. ( I ) MeRIP-qRT-PCR. RNA from NiV-infected Vero cells was immunoprecipitated with anti-m6A antibodies, followed by strand-specific qRT-PCR for NiV mRNA. Data are mean ± SEM ( n = 3); ** p < 0.01, unpaired Student’s t -test. ( J ) MeRIP-qRT-PCR analysis of m6A-modified viral RNA in lung tissues of NiV-infected hamsters. Total RNA was extracted from lung tissue, immunoprecipitated using anti-m6A antibodies, and subjected to qRT-PCR targeting NiV mRNA. Data are presented as mean ± SEM ( n = 4); * p < 0.05, unpaired Student’s t -test with Welch’s correction.

    Journal: Viruses

    Article Title: Strain-Divergent m6A Landscapes Modulate Nipah Virus Replication and METTL3 Inhibition Attenuates Virulence

    doi: 10.3390/v17060831

    Figure Lengend Snippet: Positive correlation between m6A modification levels and replication capacity of NiV strains. ( A ) NiV RNA copy numbers in supernatants collected at various time points post-infection were quantified by absolute qPCR. Data are means G SEMs ( n = 3). ** p < 0.01, two-way ANOVA with Holm–Sidak’s multiple comparisons test. ( B ) Analysis of published data comparing the impact of NiV-M and NiV-B strains on hamster survival under identical treatment conditions. ( C ) UHPLC-MS/MS analysis of NiV RNA. Viral RNA from supernatants of NiV-infected Vero cells was purified using Dynabeads and analyzed by UHPLC-MS/MS. The y -axis shows m6A as a percentage of total adenosines. ( D ) MeRIP-qRT-PCR analysis. RNA from NiV-infected Vero cells was immunoprecipitated with anti-m6A antibodies, followed by strand-specific qRT-PCR to detect the NiV negative-strand genome. Data are presented as means ± SEMs ( n = 3). ** p < 0.01, unpaired Student’s t -tests. ( E ) MeRIP-Seq. Fragmented viral RNA from the supernatant of NiV-infected Vero cells was immunoprecipitated with an m6A-specific antibody and analyzed by next-generation sequencing. Methylation coverage of input and MeRIP-enriched NiV RNA is shown in blue and red, respectively. ( F ) Nanopore DRS analysis of NiV-M and NiV-B mRNAs from infected Vero cells. The y -axis shows m6A methylation probability at each A site; only sites with >95% probability are shown. Shared m6A sites between both strains are marked in red, while NiV-M- and NiV-B-specific sites are highlighted in green and yellow, respectively. ( G , H ) Bioinformatics analysis of m6A distribution and conserved motifs in NiV-M and NiV-B mRNAs. ( I ) MeRIP-qRT-PCR. RNA from NiV-infected Vero cells was immunoprecipitated with anti-m6A antibodies, followed by strand-specific qRT-PCR for NiV mRNA. Data are mean ± SEM ( n = 3); ** p < 0.01, unpaired Student’s t -test. ( J ) MeRIP-qRT-PCR analysis of m6A-modified viral RNA in lung tissues of NiV-infected hamsters. Total RNA was extracted from lung tissue, immunoprecipitated using anti-m6A antibodies, and subjected to qRT-PCR targeting NiV mRNA. Data are presented as mean ± SEM ( n = 4); * p < 0.05, unpaired Student’s t -test with Welch’s correction.

    Article Snippet: Reverse transcription was conducted with ABScript III RT Master Mix for qPCR with gDNA Remover (ABclonal, Wuhan, China). qRT-PCR was performed using Hieff ® qPCR SYBR GreenMaster Mix (No Rox) (Yeasen, Shanghai, China) on a CFX Connect Real-Time system (Bio-Rad).

    Techniques: Modification, Infection, Tandem Mass Spectroscopy, Purification, Quantitative RT-PCR, Immunoprecipitation, Next-Generation Sequencing, Methylation

    METTL3 and ALKBH5 mediate the methylation and demethylation of NiV RNA m6A. ( A , B , E ) Western blot detection of the expression of the corresponding proteins in Vero cells treated with shMETTL3, pFlag-METTL3, pFlag-FTO, and pFlag-ALKBH5. ( C , D ) MeRIP-qRT-PCR detection of the m6A modification levels of viral RNA in NiV-infected Vero cells with METTL3 knockdown ( C ) and overexpressed ( D ). ( C ) Data are mean ± SEM ( n = 3); ** p < 0.01, one-way ANOVA with Dunnett’s multiple comparisons test. ( D ) Data are mean ± SEM ( n = 3); ** p < 0.01, unpaired Student’s t -test. ( F , G ) MeRIP-qRT-PCR detection of m6A modification levels of NiV P or NiV F RNA in pNiV-P or NiV-F-transfected Vero cells with overexpression of METTL3, ALKBH5, or FTO. Data are mean ± SEM ( n = 3); ** p < 0.01, one-way ANOVA with Dunnett’s multiple comparisons test. ( H – J ) Formaldehyde-RIP-qPCR. Vero cells transfected with NiV P, G, or L were crosslinked with formaldehyde, lysed, and subjected to immunoprecipitation (IP) using anti-METTL3 or ALKBH5 antibodies, or IgG as a control, followed by quantification via qRT-PCR. Unpaired Student’s t -test was performed, and data are presented as the means ± SEM ( n = 3). ** p < 0.01.

    Journal: Viruses

    Article Title: Strain-Divergent m6A Landscapes Modulate Nipah Virus Replication and METTL3 Inhibition Attenuates Virulence

    doi: 10.3390/v17060831

    Figure Lengend Snippet: METTL3 and ALKBH5 mediate the methylation and demethylation of NiV RNA m6A. ( A , B , E ) Western blot detection of the expression of the corresponding proteins in Vero cells treated with shMETTL3, pFlag-METTL3, pFlag-FTO, and pFlag-ALKBH5. ( C , D ) MeRIP-qRT-PCR detection of the m6A modification levels of viral RNA in NiV-infected Vero cells with METTL3 knockdown ( C ) and overexpressed ( D ). ( C ) Data are mean ± SEM ( n = 3); ** p < 0.01, one-way ANOVA with Dunnett’s multiple comparisons test. ( D ) Data are mean ± SEM ( n = 3); ** p < 0.01, unpaired Student’s t -test. ( F , G ) MeRIP-qRT-PCR detection of m6A modification levels of NiV P or NiV F RNA in pNiV-P or NiV-F-transfected Vero cells with overexpression of METTL3, ALKBH5, or FTO. Data are mean ± SEM ( n = 3); ** p < 0.01, one-way ANOVA with Dunnett’s multiple comparisons test. ( H – J ) Formaldehyde-RIP-qPCR. Vero cells transfected with NiV P, G, or L were crosslinked with formaldehyde, lysed, and subjected to immunoprecipitation (IP) using anti-METTL3 or ALKBH5 antibodies, or IgG as a control, followed by quantification via qRT-PCR. Unpaired Student’s t -test was performed, and data are presented as the means ± SEM ( n = 3). ** p < 0.01.

    Article Snippet: Reverse transcription was conducted with ABScript III RT Master Mix for qPCR with gDNA Remover (ABclonal, Wuhan, China). qRT-PCR was performed using Hieff ® qPCR SYBR GreenMaster Mix (No Rox) (Yeasen, Shanghai, China) on a CFX Connect Real-Time system (Bio-Rad).

    Techniques: Methylation, Western Blot, Expressing, Quantitative RT-PCR, Modification, Infection, Knockdown, Transfection, Over Expression, Immunoprecipitation, Control

    STM2457 as a potential antiviral drug against NiV. ( A , B ) CCK8 assay to evaluate the effect of different concentrations of STM2457 and DAA on cell viability. ( C – F ) Vero cells treated with STM2457 or DAA, infected with NiV-M or -B, were collected 48 h later, and NiV RNA copy number in the supernatant was quantified by qPCR. Data are mean ± SEM ( n = 3); ** p < 0.01, * p < 0.05, ns, no significance ( p > 0.05), one-way ANOVA with Dunnett’s multiple comparisons test. ( G ) Dose–response analysis of STM2457 in Vero cells infected with NiV-M and NiV-B; EC 50 values were determined. ( H ) Hamster were administered 20 mg/kg STM2457 via intraperitoneal injection every other day, and body weight was monitored daily. ( I ) qPCR quantification of NiV RNA copies in lung and spleen tissues of hamsters following STM2457 treatment. Data are presented as mean ± SEM ( n = 6); * p < 0.05, unpaired Student’s t -test with Welch’s correction.

    Journal: Viruses

    Article Title: Strain-Divergent m6A Landscapes Modulate Nipah Virus Replication and METTL3 Inhibition Attenuates Virulence

    doi: 10.3390/v17060831

    Figure Lengend Snippet: STM2457 as a potential antiviral drug against NiV. ( A , B ) CCK8 assay to evaluate the effect of different concentrations of STM2457 and DAA on cell viability. ( C – F ) Vero cells treated with STM2457 or DAA, infected with NiV-M or -B, were collected 48 h later, and NiV RNA copy number in the supernatant was quantified by qPCR. Data are mean ± SEM ( n = 3); ** p < 0.01, * p < 0.05, ns, no significance ( p > 0.05), one-way ANOVA with Dunnett’s multiple comparisons test. ( G ) Dose–response analysis of STM2457 in Vero cells infected with NiV-M and NiV-B; EC 50 values were determined. ( H ) Hamster were administered 20 mg/kg STM2457 via intraperitoneal injection every other day, and body weight was monitored daily. ( I ) qPCR quantification of NiV RNA copies in lung and spleen tissues of hamsters following STM2457 treatment. Data are presented as mean ± SEM ( n = 6); * p < 0.05, unpaired Student’s t -test with Welch’s correction.

    Article Snippet: Reverse transcription was conducted with ABScript III RT Master Mix for qPCR with gDNA Remover (ABclonal, Wuhan, China). qRT-PCR was performed using Hieff ® qPCR SYBR GreenMaster Mix (No Rox) (Yeasen, Shanghai, China) on a CFX Connect Real-Time system (Bio-Rad).

    Techniques: CCK-8 Assay, Infection, Injection